| 401 | Herman Miller Inc. | $180,748,569 | 8,298 | 65 |
| 402 | Bronx Defenders | $180,624,530 | 453 | 6 |
| 403 | Metroexpress Services Inc | $179,695,303 | 1,217 | 3 |
| 404 | Neelam Construction Corp | $178,850,372 | 1,944 | 8 |
| 405 | 34TH Street Partnership Inc | $178,629,560 | 38 | 2 |
| 406 | Community Partnership Charter School Education Corporation | $178,514,549 | 516 | 2 |
| 407 | B Above Worldwide Institute Inc | $178,381,321 | 2,957 | 2 |
| 408 | Brooklyn Bridge Park Corp | $177,667,160 | 44 | 1 |
| 409 | Northrop Grumman Systems Corporation | $177,287,846 | 691 | 6 |
| 410 | Regional Aid for Interim Needs Inc | $175,524,427 | 3,695 | 2 |
| 411 | Vexcel Corporation | $174,709,455 | 90 | 4 |
| 412 | Samaritan Village Inc | $174,698,491 | 619 | 6 |
| 413 | New Alternatives for Children Inc | $174,572,622 | 2,390 | 5 |
| 414 | East Side House Inc | $174,124,017 | 6,093 | 9 |
| 415 | Cellco Partnership a Verizon Wireless | $173,712,166 | 4,765 | 31 |
| 416 | Intergraph Corporation | $173,566,230 | 146 | 3 |
| 417 | Chemtall Inc. | $173,300,519 | 4,799 | 1 |
| 418 | Vocational Instruction Project Community Services Inc | $173,114,831 | 1,087 | 4 |
| 419 | Tek Systems Inc | $172,489,359 | 5,620 | 17 |
| 420 | Litehouse Builders, Inc. | $172,221,576 | 1,451 | 4 |
| 421 | Compulink Technologies Inc | $171,419,732 | 8,948 | 103 |
| 422 | Police Athletic League Inc | $171,285,336 | 4,615 | 7 |
| 423 | Purvis Systems, Incorporated | $171,185,097 | 3,083 | 3 |
| 424 | Gartner Inc | $171,171,606 | 2,152 | 35 |
| 425 | Voice Charter School | $170,620,422 | 271 | 2 |
| 426 | Catholic Guardian Society and Home Bureau | $170,324,928 | 1,851 | 3 |
| 427 | Acs System Associates, Inc | $170,048,338 | 1,764 | 15 |
| 428 | Aguila, Inc. | $169,976,195 | 634 | 2 |
| 429 | JLJ IV Enterprises Inc | $169,695,652 | 1,605 | 4 |
| 430 | Currier Mccabe & Associates Inc | $168,716,596 | 3,873 | 19 |
| 431 | Empire Control Abatement Inc. | $167,045,541 | 4,149 | 12 |
| 432 | The Child Center of Ny | $167,010,663 | 6,108 | 7 |
| 433 | Growing Up Green Charter School | $166,706,476 | 244 | 2 |
| 434 | Northeast Remsco Construction Inc | $166,682,439 | 741 | 3 |
| 435 | Lanmark Group, Inc. | $166,512,458 | 2,081 | 9 |
| 436 | D & S Restoration, Inc | $166,371,527 | 5,792 | 11 |
| 437 | Delphi Plumbing & Heating Inc | $165,925,425 | 968 | 9 |
| 438 | Denville Line Painting, Inc. | $165,659,211 | 996 | 3 |
| 439 | East Harlem Village Academy Charter School | $165,314,459 | 267 | 1 |
| 440 | Independent Temperature Contro | $165,304,853 | 1,221 | 2 |
| 441 | Kipp Nyc Public Charter Schools | $165,238,928 | 287 | 2 |
| 442 | Chinese American Planning Council Inc | $164,754,027 | 4,393 | 11 |
| 443 | T-Mobile USA Inc | $164,727,634 | 2,710 | 8 |
| 444 | Montefiore Medical Center | $163,867,774 | 1,635 | 13 |
| 445 | First American Title Insurance Company | $163,830,387 | 45 | 4 |
| 446 | Atlantic Escorts, Inc. | $163,810,183 | 93 | 1 |
| 447 | The Opportunity Charter School | $163,261,065 | 206 | 1 |
| 448 | Cde Air Conditioning Co Inc | $163,014,312 | 2,161 | 15 |
| 449 | Skanska USA Building Inc | $162,601,536 | 148 | 4 |
| 450 | Vanderbilt Associates Owner LP | $162,407,833 | 378 | 1 |