| 251 | Mill Basin Bridge Constructors LLC | $259,850,943 | 320 | 1 |
| 252 | Central Mechanical Systems Inc | $258,483,939 | 1,335 | 3 |
| 253 | Correctional Medical Associates of New York PC | $258,444,288 | 167 | 1 |
| 254 | Conti of New York LLC | $258,099,069 | 191 | 2 |
| 255 | Eastern Shipbuilding Group Inc | $257,995,190 | 265 | 2 |
| 256 | Selfhelp Community Services, Inc. | $257,842,816 | 3,998 | 5 |
| 257 | Kipp Academy Charter School | $257,811,144 | 333 | 3 |
| 258 | MFM Contracting Corp. | $257,598,675 | 3,620 | 7 |
| 259 | H.e.l.p. Social Service Corporation | $257,121,434 | 1,392 | 3 |
| 260 | Highland Park Community Development Corp. | $256,861,396 | 622 | 3 |
| 261 | Teri Nichols Institutional Food Merchants LLC | $255,526,507 | 2,172 | 3 |
| 262 | Coney Island Preparatory Public Charter School | $255,331,631 | 287 | 3 |
| 263 | Children's Rescue Fund - Icahn House | $255,328,655 | 508 | 1 |
| 264 | Accenture LLP | $254,787,708 | 1,780 | 18 |
| 265 | John P Picone Inc | $254,587,940 | 574 | 4 |
| 266 | Amcc Corp | $254,316,716 | 1,666 | 1 |
| 267 | Brookfield Construction Assoc. LLC | $253,545,332 | 309 | 2 |
| 268 | PHS Medical Services PC | $253,302,384 | 132 | 1 |
| 269 | Eic.associates Inc | $251,882,858 | 1,524 | 5 |
| 270 | Center for Alternative Sentencing and Employment Services in | $249,402,590 | 1,786 | 10 |
| 271 | University Settlement Society of New York | $248,983,150 | 6,417 | 10 |
| 272 | Hunter Roberts Construction Group, LLC | $244,738,289 | 374 | 5 |
| 273 | Aguila Inc | $244,713,000 | 839 | 3 |
| 274 | Laws Construction Corp. | $243,910,804 | 1,664 | 6 |
| 275 | Hyde Leadership Charter School | $243,173,984 | 200 | 2 |
| 276 | Essey Group LLC | $241,796,920 | 23,009 | 10 |
| 277 | United Metro Energy Corp | $241,568,328 | 1,422 | 6 |
| 278 | The Salvation Army | $241,236,121 | 3,467 | 10 |
| 279 | Queens Community House, Inc. | $240,586,460 | 7,125 | 14 |
| 280 | Goodwill Industries of Greater Ny & Northern New Jersey Inc | $240,019,546 | 9,449 | 8 |
| 281 | Central Park Conservancy Inc | $239,196,368 | 203 | 2 |
| 282 | Cgi Technologies and Solutions Inc | $238,958,248 | 1,949 | 9 |
| 283 | Crown Castle Fiber LLC | $238,593,158 | 3,695 | 7 |
| 284 | Edwin Gould Services for Children & Families | $237,542,043 | 2,830 | 8 |
| 285 | Spruce Technology Inc | $236,942,471 | 5,049 | 35 |
| 286 | Housing Partners of New York Inc a Housing Bridge | $236,535,005 | 580 | 1 |
| 287 | The Williamsburg Charter School | $236,195,593 | 210 | 1 |
| 288 | Five Star Electric Corp. | $235,184,746 | 1,655 | 8 |
| 289 | Adc Construction L.L.C | $234,630,388 | 1,678 | 3 |
| 290 | C&L Contracting Corp | $231,162,335 | 2,042 | 7 |
| 291 | Tully Environmental Inc | $229,202,670 | 1,288 | 7 |
| 292 | Watershed Agrig. Council | $228,753,714 | 215 | 1 |
| 293 | Sports & Arts in Schools Foundation, Inc. | $228,506,113 | 8,968 | 3 |
| 294 | Welkin Mechanical | $228,307,847 | 2,124 | 7 |
| 295 | Lutheran Social Services of Metropolitan New York Inc | $227,673,350 | 3,016 | 5 |
| 296 | STV Inc. | $227,122,405 | 18,430 | 17 |
| 297 | Harlem Children's Zone, Inc. Promise Academy | $226,040,189 | 274 | 1 |
| 298 | The Community Preservation Corp | $225,753,015 | 448 | 2 |
| 299 | Riseboro Community Partnership Inc | $225,381,259 | 4,718 | 14 |
| 300 | Whitsons Food Service Bronx Corp | $225,221,864 | 4,593 | 5 |