| 301 | Martin County Bocc | $474,416,078 | 3,815 | 17 |
| 302 | Walton County School BoardGov | $472,541,789 | 7,417 | 12 |
| 303 | State College of Florida, Manat | $470,513,647 | 2,292 | 15 |
| 304 | City of Fort MyersGov | $465,473,285 | 7,779 | 33 |
| 305 | Columbia County Bocc | $465,232,517 | 4,725 | 21 |
| 306 | Okaloosa County School BoardGov | $463,483,755 | 3,043 | 14 |
| 307 | Southland Construction, Inc. | $463,362,111 | 1,304 | 3 |
| 308 | Truecore Behavioral Solutions, | $462,456,407 | 7,077 | 6 |
| 309 | Family Support Services of Sunc | $461,078,442 | 443 | 2 |
| 310 | Jacksonville Port AuthorityGov | $461,073,092 | 409 | 15 |
| 311 | Conduent State & Local Solution | $460,498,113 | 948 | 6 |
| 312 | Pride Enterprises | $459,692,958 | 571,960 | 45 |
| 313 | Apac-Southeast, Inc | $458,701,455 | 1,816 | 1 |
| 314 | Charles Schwab Trust Company | $454,800,153 | 374 | 1 |
| 315 | Florida Coalition Against Domes | $451,212,499 | 1,424 | 9 |
| 316 | Sumter County School BoardGov | $450,528,324 | 9,466 | 17 |
| 317 | Putnam County Bocc | $450,332,917 | 5,908 | 25 |
| 318 | Sacyr Construccion Sa | $450,259,315 | 480 | 1 |
| 319 | City of MiramarGov | $447,404,515 | 4,556 | 23 |
| 320 | City of Boca RatonGov | $447,303,755 | 2,853 | 20 |
| 321 | State of Florida 457(b)Gov | $445,169,742 | 208 | 1 |
| 322 | Clerk of the Circuit Court | $444,650,126 | 3,173 | 18 |
| 323 | Sallie Mae Service Center | $440,783,599 | 493 | 1 |
| 324 | City of PensacolaGov | $439,484,382 | 5,742 | 26 |
| 325 | Pcl/Archer Western, a Joint Ven | $439,458,263 | 172 | 1 |
| 326 | Board of County Commissioners L | $438,568,700 | 3,918 | 24 |
| 327 | Nassau County Bocc | $438,029,409 | 5,692 | 20 |
| 328 | Embrace Families Community Base | $431,929,068 | 805 | 3 |
| 329 | City of SarasotaGov | $429,880,423 | 5,449 | 26 |
| 330 | Voya Retirement Insurance and a | $425,199,055 | 568 | 3 |
| 331 | Federal Emergency Management Ag | $425,006,387 | 644 | 5 |
| 332 | Ashbritt, Inc. | $424,145,122 | 277 | 3 |
| 333 | Seminole County Coalition for | $421,074,697 | 903 | 2 |
| 334 | Insight Public Sector, Inc. | $419,768,744 | 30,609 | 44 |
| 335 | Gulf Coast State College | $417,801,013 | 3,950 | 21 |
| 336 | Agency for Health Care Admin.Gov | $415,648,378 | 12 | 2 |
| 337 | City of Key WestGov | $410,527,249 | 4,678 | 26 |
| 338 | Jr. Davis Construction Company, | $410,100,114 | 443 | 2 |
| 339 | P & S Paving, Inc. | $409,302,592 | 1,706 | 3 |
| 340 | Condotte/De Moya JV, LLC | $409,065,881 | 237 | 1 |
| 341 | Polk State College | $407,606,413 | 2,189 | 18 |
| 342 | City of KissimmeeGov | $405,326,586 | 4,621 | 24 |
| 343 | Agi de Moya Joint Venture | $402,406,164 | 354 | 1 |
| 344 | City of Miami BeachGov | $401,988,069 | 4,710 | 32 |
| 345 | First Federal Bank | $401,701,560 | 228 | 8 |
| 346 | Harris Corporation | $400,662,396 | 3,317 | 23 |
| 347 | Cone & Graham, Inc. | $400,631,251 | 1,277 | 3 |
| 348 | Martin County Board of County C | $400,392,789 | 4,559 | 23 |
| 349 | Calhoun County School BoardGov | $396,586,554 | 5,871 | 13 |
| 350 | Orange County Clerk of Circuit | $394,649,796 | 377 | 9 |