| 251 | St Lucie County Bocc | $574,692,518 | 5,525 | 22 |
| 252 | Dell Marketing L.P. | $574,217,684 | 101,523 | 46 |
| 253 | Sumter County Bocc | $571,729,009 | 5,707 | 22 |
| 254 | Triumph Gulf Coast | $569,419,274 | 9 | 3 |
| 255 | Highlands County Bocc | $568,660,542 | 5,530 | 24 |
| 256 | Florida Tourism Industry Market | $566,398,801 | 481 | 18 |
| 257 | Electronic Data Systems | $565,375,474 | 1,780 | 19 |
| 258 | State of FloridaGov | $563,450,106 | 754 | 21 |
| 259 | Deloitte Consulting, LLP | $558,422,618 | 2,166 | 13 |
| 260 | Geo Secure Services LLC | $557,432,557 | 808 | 2 |
| 261 | Metric Engineering, Inc. | $553,524,664 | 13,232 | 2 |
| 262 | Fla International Univ Eft | $553,289,670 | 730 | 4 |
| 263 | The Early Learning Coalition of | $553,189,881 | 1,109 | 6 |
| 264 | City of Port St.lucieGov | $552,604,361 | 1,136 | 1 |
| 265 | Community Based Care of Central | $552,467,668 | 1,054 | 6 |
| 266 | The Middlesex Corporation | $546,286,976 | 1,028 | 4 |
| 267 | Management & Training Corporait | $544,302,814 | 854 | 3 |
| 268 | Tidal Basin Government Consulti | $543,970,175 | 409 | 6 |
| 269 | College of Central Florida | $539,299,360 | 3,209 | 23 |
| 270 | Hernando County Bocc | $538,734,328 | 4,824 | 26 |
| 271 | L3harris Technologies Inc | $535,158,804 | 891 | 19 |
| 272 | Miami Dade County Florida | $534,120,870 | 4,198 | 27 |
| 273 | Alachua County Bocc | $532,106,483 | 2,525 | 18 |
| 274 | City of HollywoodGov | $529,869,049 | 8,022 | 27 |
| 275 | City of Pembroke PinesGov | $527,349,579 | 6,343 | 27 |
| 276 | Pasco Hernando State College | $516,716,193 | 4,136 | 12 |
| 277 | Ounce of Prevention Fund of FL | $513,099,913 | 1,532 | 9 |
| 278 | Dbi Services, LLC | $512,801,161 | 2,009 | 3 |
| 279 | CF Regional Workforce Dev Board | $512,649,095 | 4,104 | 6 |
| 280 | Florida Southwestern State Coll | $508,657,022 | 3,826 | 18 |
| 281 | CDR Maguire, Inc. | $505,922,239 | 1,002 | 5 |
| 282 | Hillsborough County Aviation | $503,454,503 | 2,207 | 18 |
| 283 | Florida Dept of Health Wic | $502,836,308 | 513 | 1 |
| 284 | Holmes County School BoardGov | $499,817,311 | 4,680 | 14 |
| 285 | Transcore L P | $498,769,551 | 2,537 | 1 |
| 286 | Correct Care, LLC | $498,559,138 | 414 | 3 |
| 287 | City of West Palm BeachGov | $497,658,208 | 15,770 | 31 |
| 288 | Cheney Bros Inc | $497,066,033 | 35,514 | 20 |
| 289 | Astaldi Construction Corp. | $494,802,244 | 914 | 1 |
| 290 | Northwest Florida State College | $492,835,428 | 2,703 | 16 |
| 291 | Early Learning Coalition of Big | $490,134,929 | 857 | 6 |
| 292 | Greater Orlando Aviation Author | $485,335,884 | 836 | 23 |
| 293 | Compbenefits Dental & Vision Co | $484,526,643 | 522 | 1 |
| 294 | Early Learning Coalition of Nor | $482,352,918 | 623 | 1 |
| 295 | First Foundation Bank | $482,328,379 | 14 | 4 |
| 296 | City of North PortGov | $480,436,532 | 4,188 | 20 |
| 297 | City of LakelandGov | $479,219,317 | 20,830 | 33 |
| 298 | St Johns River Comm College | $478,910,972 | 2,812 | 11 |
| 299 | Bradford County School BoardGov | $477,418,079 | 6,338 | 20 |
| 300 | Baker County School Board1 | $474,508,656 | 5,107 | 11 |