| 551 | Lee County Board of County Comm | $217,717,811 | 7,508 | 29 |
| 552 | Leon County Bocc | $217,205,437 | 2,123 | 20 |
| 553 | Macro Oil Company | $216,714,230 | 655 | 3 |
| 554 | Northwest Florida Water Managem | $215,975,776 | 660 | 14 |
| 555 | Volkert, Inc. | $215,717,110 | 5,312 | 2 |
| 556 | Edison State College | $215,380,739 | 2,054 | 10 |
| 557 | Citrus County, Florida | $215,154,916 | 3,254 | 22 |
| 558 | Hillsborough County Sheriff's O | $215,107,701 | 3,080 | 19 |
| 559 | Lee County Port AuthorityGov | $214,364,834 | 1,122 | 16 |
| 560 | Orion Marine Construction, Inc. | $214,231,765 | 381 | 2 |
| 561 | Embry-Riddle Aeronautical Unive | $214,128,050 | 3,823 | 12 |
| 562 | Geo Corrections Holdings, Inc. | $214,099,711 | 255 | 4 |
| 563 | Bergeron Land Development, Inc | $213,240,188 | 585 | 4 |
| 564 | City of Coral GablesGov | $212,397,841 | 2,367 | 24 |
| 565 | Sls-Wsp JV LLC | $211,366,876 | 184 | 1 |
| 566 | Early Learning Coalition of Flo | $209,688,016 | 700 | 4 |
| 567 | City of DoralGov | $208,720,433 | 1,998 | 20 |
| 568 | City of Winter ParkGov | $207,884,905 | 3,071 | 20 |
| 569 | Family Central, Inc. | $207,683,963 | 657 | 6 |
| 570 | Bellsouth Telecommunications, I | $206,467,193 | 12,256 | 20 |
| 571 | Access Restoration Services, US | $206,455,545 | 51 | 1 |
| 572 | Wexford Health Sources Inc. | $205,786,064 | 296 | 4 |
| 573 | Horne LLP | $205,612,204 | 392 | 8 |
| 574 | Conduent State & Local | $205,527,681 | 26 | 3 |
| 575 | Daytona Beach Community CollegeGov | $205,325,965 | 973 | 10 |
| 576 | Careersource Broward | $204,547,600 | 3,742 | 5 |
| 577 | Phoenix Construction Services, | $203,260,667 | 552 | 1 |
| 578 | Vista Healthplan, Inc. | $203,249,763 | 30 | 2 |
| 579 | Sequel Tsi of Florida, LLC | $203,173,862 | 3,363 | 2 |
| 580 | Associated Marine Institutes | $203,153,245 | 2,645 | 6 |
| 581 | Abml. LLC | $202,905,888 | 48 | 1 |
| 582 | Baker County School BoardGov | $202,510,888 | 2,863 | 11 |
| 583 | Post, Buckley, Schuh & Jerniga | $202,157,013 | 3,764 | 4 |
| 584 | Broward County Clerk of Court | $201,915,802 | 544 | 11 |
| 585 | Hillsborough Co Clerk of Court | $201,713,511 | 67 | 4 |
| 586 | Parsons Brinckerhoff Inc. | $200,690,904 | 3,148 | 1 |
| 587 | City of LauderhillGov | $200,504,922 | 2,242 | 21 |
| 588 | Florida Gas Tranmission Co, LLC | $200,267,004 | 106 | 3 |
| 589 | Archer Western Contractors-Rail | $198,567,946 | 72 | 1 |
| 590 | City of Palm Beach GardensGov | $198,425,079 | 4,247 | 18 |
| 591 | Ahca Medicaid Payments Anly Eft | $198,000,000 | 2 | 1 |
| 592 | Madison County Bocc | $196,172,385 | 5,645 | 24 |
| 593 | Sharma Eminent Domain Lawyers | $195,315,053 | 94 | 1 |
| 594 | City of TamaracGov | $194,426,397 | 1,549 | 18 |
| 595 | City of VeniceGov | $193,861,229 | 4,327 | 21 |
| 596 | City of Altamonte SpringsGov | $193,776,330 | 1,719 | 19 |
| 597 | City of North Miami BeachGov | $193,315,945 | 2,838 | 21 |
| 598 | Atkinsrealis USA Inc | $193,291,825 | 1,494 | 4 |
| 599 | Medimpact Healthcare Systems, I | $193,018,580 | 382 | 1 |
| 600 | US Bank | $192,770,735 | 198 | 3 |