| 601 | Memorial Healthcare Group, Inc. | $192,537,105 | 15,893 | 8 |
| 602 | Lead Engineering Contractors | $191,990,891 | 559 | 1 |
| 603 | Louis Berger Hawthorne Services | $191,401,779 | 645 | 3 |
| 604 | Avmed Inc | $190,834,791 | 249 | 7 |
| 605 | St Johns County Bocc | $190,457,632 | 947 | 11 |
| 606 | City of Port OrangeGov | $190,321,869 | 4,615 | 20 |
| 607 | Citrus Research and Development | $189,798,115 | 414 | 3 |
| 608 | Lemoine CDR Logistics, LLC | $188,225,591 | 17 | 1 |
| 609 | Highway Safety Devices, Inc | $188,095,208 | 1,100 | 1 |
| 610 | Gannett Fleming, Inc. | $188,064,925 | 7,400 | 4 |
| 611 | City of NaplesGov | $188,020,494 | 3,801 | 20 |
| 612 | Gaylord Merlin Ludovici & Diaz | $187,859,668 | 467 | 3 |
| 613 | City of North MiamiGov | $186,888,651 | 4,952 | 20 |
| 614 | Traffic Control Devices, Inc. | $186,313,375 | 1,626 | 3 |
| 615 | Wells Fargo Bank, N.a. | $185,300,200 | 1,685 | 21 |
| 616 | KLC Consulting, Inc. | $185,273,197 | 11,516 | 24 |
| 617 | City of WestonGov | $185,005,150 | 1,209 | 11 |
| 618 | Gulf County Bocc | $183,872,390 | 4,922 | 24 |
| 619 | Brevard Workforce Development | $182,958,980 | 2,817 | 7 |
| 620 | Bradford County Bocc | $182,133,337 | 4,874 | 22 |
| 621 | City of Port St LucieGov | $182,024,306 | 3,766 | 30 |
| 622 | Transfield Services Infrastruct | $181,882,633 | 762 | 1 |
| 623 | Clark Construction Group | $180,304,314 | 69 | 1 |
| 624 | Careersource Florida, Inc. | $179,999,294 | 1,325 | 16 |
| 625 | City of TitusvilleGov | $179,925,051 | 4,553 | 24 |
| 626 | Pepper Contracting Services, in | $179,921,601 | 654 | 2 |
| 627 | Calhoun County Bocc | $179,593,053 | 5,169 | 21 |
| 628 | Washington County CommissionGov | $179,251,199 | 2,966 | 18 |
| 629 | Granite Construction Company | $178,268,315 | 214 | 6 |
| 630 | Pinellas County Sheriff's Offic | $177,572,383 | 1,637 | 17 |
| 631 | Department of Financial ServiceGov | $177,305,491 | 558 | 11 |
| 632 | City of Riviera BeachGov | $177,089,538 | 3,677 | 24 |
| 633 | City of Winter HavenGov | $176,854,059 | 5,739 | 25 |
| 634 | Suwannee River Water | $176,606,019 | 639 | 15 |
| 635 | Palm Beach County Board of Coun | $176,288,810 | 7,136 | 28 |
| 636 | Gulf Coast Treatment Center, in | $175,191,487 | 2,765 | 4 |
| 637 | Eqhealth Solutions, Inc | $175,089,198 | 500 | 2 |
| 638 | Orange CountyGov | $175,066,663 | 1,612 | 22 |
| 639 | Lee Memorial Health System | $174,963,254 | 13,563 | 20 |
| 640 | Office Depot | $174,777,588 | 1,201,606 | 46 |
| 641 | First American Title | $174,680,285 | 111 | 4 |
| 642 | Pinellas County Board of CountyGov | $174,443,922 | 2,402 | 24 |
| 643 | City of Oakland ParkGov | $174,163,298 | 4,139 | 21 |
| 644 | Emerald Coast Utilities Authori | $173,765,567 | 4,072 | 21 |
| 645 | VMS Inc | $173,610,026 | 734 | 2 |
| 646 | City of ClermontGov | $173,346,083 | 4,600 | 19 |
| 647 | City of GreenacresGov | $172,773,511 | 3,296 | 20 |
| 648 | Biltmore Construction Co. Inc. | $172,737,755 | 1,784 | 13 |
| 649 | City of St PetersburgGov | $172,659,005 | 6,324 | 25 |
| 650 | Stetson University | $171,430,835 | 3,936 | 21 |