| 501 | Kiewit Southern Co. | $251,672,454 | 486 | 1 |
| 502 | Communities Connected for Kids, | $250,399,693 | 826 | 1 |
| 503 | City of JacksonvilleGov | $248,584,781 | 6,400 | 36 |
| 504 | Raytheon Company | $248,557,530 | 1,592 | 5 |
| 505 | Hamilton County School BoardGov | $247,998,650 | 4,552 | 12 |
| 506 | Hendry County Bocc | $247,755,794 | 5,557 | 24 |
| 507 | City of Punta GordaGov | $247,601,862 | 3,515 | 18 |
| 508 | Gothams LLC | $246,380,444 | 155 | 2 |
| 509 | Village of WellingtonGov | $245,563,337 | 1,907 | 20 |
| 510 | Gilchrist County School Distric | $245,497,697 | 3,253 | 10 |
| 511 | Southwest Florida Water MGT DST | $245,312,021 | 1,051 | 11 |
| 512 | City of BradentonGov | $244,253,125 | 4,749 | 25 |
| 513 | Consor Engineers, LLC | $244,234,629 | 5,209 | 5 |
| 514 | South Florida Community Care Ne | $243,744,934 | 722 | 7 |
| 515 | Archer Western Construction, LL | $243,028,788 | 277 | 2 |
| 516 | Gadsden County Board of Commiss | $242,994,064 | 4,157 | 15 |
| 517 | Lake Sumter State College | $241,428,906 | 864 | 9 |
| 518 | Canaveral Port AuthorityGov | $240,778,130 | 361 | 13 |
| 519 | Duval Asphalt Products, Inc. | $239,013,805 | 1,976 | 11 |
| 520 | Town of JupiterGov | $236,727,428 | 3,340 | 19 |
| 521 | Nelnet | $235,649,609 | 656 | 2 |
| 522 | Miami-Dade County Board of | $235,600,309 | 679 | 14 |
| 523 | City of ApopkaGov | $235,592,990 | 3,873 | 24 |
| 524 | Gainwell Technologies LLC | $234,745,104 | 335 | 2 |
| 525 | Blue Cross Blue Shield of Flori | $233,495,144 | 321 | 8 |
| 526 | Kisinger Campo & Associates Cor | $233,477,906 | 3,883 | 2 |
| 527 | City of HomesteadGov | $233,067,580 | 6,470 | 22 |
| 528 | Hardee County Bocc | $233,026,356 | 5,372 | 23 |
| 529 | Medimpact Healthcare Systems | $231,476,937 | 298 | 1 |
| 530 | Innovative Emergency Management | $231,315,985 | 411 | 7 |
| 531 | School Readiness Coalition | $231,302,502 | 784 | 3 |
| 532 | City of Deerfield BeachGov | $230,015,792 | 3,155 | 21 |
| 533 | University of West Florida Eft | $228,996,927 | 2,330 | 18 |
| 534 | Centurylink | $227,801,936 | 81,554 | 43 |
| 535 | Gulf Coast Community CollegeGov | $227,356,987 | 2,443 | 20 |
| 536 | Aim Engineering & Surveying | $226,180,638 | 3,835 | 2 |
| 537 | Skanska-Ajax Paving Joint Ventu | $225,528,054 | 88 | 2 |
| 538 | City of MarathonGov | $223,640,782 | 3,329 | 20 |
| 539 | Southwest Florida Workforce de | $223,543,771 | 5,863 | 11 |
| 540 | Wakulla County Board of CountyGov | $222,872,305 | 5,324 | 25 |
| 541 | City of OcoeeGov | $222,669,835 | 2,567 | 16 |
| 542 | Elc of Southwest Florida | $222,547,890 | 283 | 3 |
| 543 | Broward County, Florida | $221,977,405 | 32,094 | 23 |
| 544 | City of Fort PierceGov | $220,747,092 | 4,772 | 24 |
| 545 | Kyra Solutions, Inc. (formerly | $218,734,086 | 15,282 | 24 |
| 546 | City of Winter GardenGov | $218,549,229 | 3,347 | 17 |
| 547 | Wells Fargo Bank Na | $218,482,983 | 674 | 16 |
| 548 | Favorite Healthcare Staffing, I | $218,193,869 | 1,924 | 3 |
| 549 | City of St CloudGov | $218,188,034 | 1,682 | 13 |
| 550 | Halifax Paving, Inc. | $218,055,324 | 1,021 | 4 |