| 1001 | England-Thims, & Miller, Inc. | $94,258,050 | 1,846 | 4 |
| 1002 | City of Live OakGov | $94,201,008 | 5,549 | 24 |
| 1003 | Perry-Mccall Construction, Inc. | $93,822,702 | 34 | 3 |
| 1004 | Northstar Contracting Group, in | $93,762,146 | 1,908 | 7 |
| 1005 | Verizon Wireless | $93,678,594 | 71,305 | 43 |
| 1006 | Convergys Customer Management G | $93,615,470 | 56 | 4 |
| 1007 | Thomas Howell Ferguson P.A. | $93,384,610 | 1,808 | 26 |
| 1008 | Balfour Beatty Construction, LL | $93,018,340 | 68 | 2 |
| 1009 | Hillsborough Transit AuthorityGov | $92,976,967 | 8,498 | 10 |
| 1010 | A&p Consulting Transportation | $92,798,333 | 2,142 | 1 |
| 1011 | Deangelo Contracting Services, | $92,734,761 | 242 | 3 |
| 1012 | City of Mount DoraGov | $92,184,262 | 3,553 | 23 |
| 1013 | Alma DDB LLC | $92,121,588 | 24 | 1 |
| 1014 | Seminole County Sheriff's Offic | $92,048,674 | 2,710 | 20 |
| 1015 | Clay Electric Cooperative, Inc. | $91,810,066 | 34,148 | 17 |
| 1016 | Mote Marine Laboratory | $91,745,315 | 2,859 | 12 |
| 1017 | Deployed Resources, LLC | $91,579,876 | 26 | 1 |
| 1018 | Broward Metropolitan Planning O | $91,443,812 | 434 | 1 |
| 1019 | Novitex Enterprise Solutions, I | $91,443,126 | 1,932 | 20 |
| 1020 | Doodie Calls Inc | $91,388,873 | 142 | 7 |
| 1021 | Blue Cross/Blue Shield | $91,293,989 | 85 | 2 |
| 1022 | Consortium of Florida Education | $91,162,824 | 139 | 4 |
| 1023 | Lafayette County Bocc | $91,160,499 | 4,509 | 23 |
| 1024 | Suwannee River Economic Council | $91,129,484 | 3,073 | 14 |
| 1025 | Traffic Control Devices Inc | $90,977,663 | 602 | 3 |
| 1026 | CSX Transportation, Inc. | $90,759,522 | 5,300 | 3 |
| 1027 | Big Bend Jobs & Education | $90,683,952 | 2,958 | 9 |
| 1028 | Orlando Utilities CommissionGov | $90,567,840 | 13,991 | 23 |
| 1029 | Ips Enterprises Inc | $90,527,480 | 47 | 2 |
| 1030 | City of Tarpon SpringsGov | $90,337,964 | 3,532 | 19 |
| 1031 | PCS Civil Inc. F/K/A Pepper Con | $90,236,763 | 257 | 1 |
| 1032 | American Express TRS | $90,235,304 | 7,872 | 18 |
| 1033 | Longview International Technolo | $89,988,974 | 74 | 2 |
| 1034 | Polk County Clerk of Court | $89,629,556 | 21,051 | 10 |
| 1035 | City of LongwoodGov | $89,583,483 | 2,800 | 19 |
| 1036 | CDW Government LLC | $89,530,959 | 33,560 | 46 |
| 1037 | Tallahassee State College | $89,488,076 | 1,140 | 15 |
| 1038 | Iem International, Inc. | $89,220,340 | 164 | 4 |
| 1039 | Kiewit Infrastructure South Co. | $89,155,838 | 389 | 2 |
| 1040 | General Building Maintenance of | $89,035,142 | 5,940 | 18 |
| 1041 | Worknet Pinellas, Inc. | $88,499,835 | 1,052 | 3 |
| 1042 | Atlantic Civil Constructors Cor | $87,859,269 | 514 | 6 |
| 1043 | Oasis Landscape Services, Inc. | $87,801,802 | 1,254 | 3 |
| 1044 | Charles Perry Partners, Inc. | $87,624,280 | 1,113 | 15 |
| 1045 | City of Atlantic BeachGov | $87,204,048 | 3,205 | 16 |
| 1046 | Idemia Identity & Security USA | $87,154,176 | 3,086 | 20 |
| 1047 | Sma Behavioral Health Services, | $86,945,575 | 12,050 | 14 |
| 1048 | Ferreira Construction Co., Inc. | $86,921,793 | 290 | 2 |
| 1049 | City of Lake MaryGov | $86,828,157 | 2,258 | 16 |
| 1050 | Children's Home Society of Flor | $86,476,614 | 8,036 | 17 |